U-CH22

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General Characteristics

Age: 78

Gender: Female

Disease Origin: Sacral

Disease Status: Recurrent + Metastatic

Metastatic Site(s): Thigh, sacrum, ilium, clavicle, lungs, peritoneum, lymph nodes, adrenal gland, and thyroid

Sample Origin: Pleural effusion

Prior Radiation: Yes

Prior Systemic Therapy: Yes (imatinib, olaparib)

Source: Kevin Mellert, University of Ulm

Notes: This cell line was developed from cells isolated from pleural effusion fluid.

Molecular Features

Cell Morphology: Mostly non-physaliferous

TBXT Expression: Positive

TBXT Localization: Nuclear

CD24 Expression:

HLA Type:

Sequence Data:

SMARCB1 Status: Presumed SMARCB1-null (poorly differentiated)

Validation Results: Validation Report

Growth Conditions

Growth Medium: (4:1) IMDM/RPMI 1640 + 10% FBS + 1% NEAA + 1% GlutaMAX

Freeze Medium: 70% complete growth medium supplemented with an additional 20% fetal bovine serum and 10% DMSO

Culture Conditions: Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing Instructions: Cell Culture Procedures

Publications

Characterized By:

  • Bette S, et al. (2024) Impact of CDK Inhibitors on TBXT Expression in Chordoma Cell Lines Including the First Stable Cell Line of a High-Grade Chordoma. Diagnostics (Basel). PubMed: 38786326.