General Characteristics
Age: 38 years
Gender: Male
Disease Origin: Sacral
Disease Status: Metastatic
Metastatic Site(s): Skin, Soft tissue
Sample Origin: Skin
Prior Radiation: No
Prior Systemic Therapy: No
Source: Kevin Mellert, Peter Moller; University of Ulm
Molecular Features
Cell Morphology: Physaliferous
TBXT Expression: Positive; 5x that of U-CH2
TBXT Localization: Nuclear
CD24 Expression: Positive; 3x that of U-CH2
HLA Type:
Sequence Data: WGS and transcriptome data available: raw sequencing data in Cavatica (Chordoma Foundation Dataset) and summary data in PedcBioportal. Contact researchteam@chordoma.org with any questions.
SMARCB1 Status: Wild-type
Validation Results: Validation Report
Growth Conditions
Growth Medium: (4:1) IMDM/RPMI 1640 + 10% FBS + 1% NEAA + 1% GlutaMAX
Freeze Medium: 70% complete growth medium supplemented with an additional 20% fetal bovine serum and 10% DMSO
Culture Conditions: Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing Instructions: Cell Culture Procedures
Publications
Characterized By:
- Jäger D, Lechel A, Tharehalli U, Seeling C, Möller P, Barth TFE, Mellert K. (2017) U-CH17P, -M and -S, a new cell culture system for tumor diversity and progression in chordoma. Int J Cancer doi: 10.1002/ijc.31161.