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JHC7

General Characteristics

Age: 61 years
Gender: Female
Disease Origin: Sacral
Disease Status: Primary
Prior Radiation: No
Prior Systemic Therapy: No
Source: Sagar Shah and Alfredo Quiñones-Hinojosa
Johns Hopkins University

Molecular Features

Cell Morphology: Physaliferous
TBXT Expression: Positive; 9x that of U-CH2 and with T amplification
TBXT Localization: Nuclear
CD24 Expression: Positive; 6x that of U-CH2
HLA Type: A24
Sequence Data: WGS, exome and transcriptome data available: raw sequencing data in Cavatica
(Chordoma Foundation Dataset) and summary data in PedcBioportal. Contact researchteam@chordoma.org with any questions.
SMARCB1 Status:
Validation Results: Validation Reports

Growth Conditions

Growth Medium: DMEM/F12+10%FBS+glutamine
Freeze Medium: 70% complete growth medium supplemented with an additional 20% fetal bovine serum and 10% DMSO
Culture Conditions: Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing Instructions: Cell Culture Procedures

Publications

Characterized By:
  • Hsu W, Mohyeldin A, Shah SR, ap Rhys CM, Johnson LF et al. (2011) Generation of chordoma celll line JHC7 and the identification of Brachyury as a novel molecular target. J Neurosurg 115(4): 760-9. PubMed: 21699479.