General Characteristics
Age: 56 years
Gender: Female
Disease Origin: Sacral
Disease Status: Recurrent, Metastatic
Metastatic Site(s): Liver, Peritoneum
Sample Origin: Peritoneum
Prior Radiation: Yes
Prior Systemic Therapy: No
Source: Francis Hornicek, Zhenfeng Duan, Massachusetts General Hospital
Molecular Features
Cell Morphology: Physaliferous
TBXT Expression: Positive; 20x that of U-CH2
TBXT Localization: Nuclear
CD24 Expression: Positive; 18x that of U-CH2
HLA Type:
Sequence Data: WGS and transcriptome data available: raw sequencing data in Cavatica (Chordoma Foundation Dataset) and summary data in PedcBioportal. Contact researchteam@chordoma.org with any questions.
SMARCB1 Status: No expression
Validation Results: Validation Report
Growth Conditions
Growth Medium: (4:1) IMDM/RPMI 1640 + 10% FBS + 1% NEAA + 1% GlutaMAX
Freeze Medium: 70% complete growth medium supplemented with an additional 20% fetal bovine serum and 10% DMSO
Culture Conditions: Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing Instructions: Cell Culture Procedures
Publications
Characterized By:
- Establishment and characterization of a novel chordoma cell line: CH22. Liu X, Nielsen GP, Rosenberg AE, Waterman PR, Yang W, Choy E, Sassi S, Yang S, Harmon DC, Yang C, Schwab JH, Kobayashi E, Mankin HJ, Xavier R, Weissleder R, Duan Z, Hornicek FJ. J Orthop Res. 2012 Oct;30(10):1666-73. doi: 10.1002/jor.22113.