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General Characteristics

Age:56 years
Disease Origin:Sacral
Disease Status:Recurrent, Metastatic
Metastatic Site(s):Liver, Peritoneum
Sample Origin:Peritoneum
Source:Francis Hornicek, Zhenfeng Duan
Massachusetts General Hospital

Molecular Features

Cell morphology:Physaliferous
T expression:Positive; 19.5x that of U-CH2
T localization:Nuclear
CD24 expression:Positive; 18.2x that of U-CH2
SMARCB1 expression:No
HLA Type:
Chromosomal abnormalities:Uncharacterized
Sequence data:WGS and Transcriptome data available contact
Validation results:Validation Report

Growth Conditions

Growth medium:RPMI +10% FBS
Freeze medium:70% complete growth medium supplemented with an additional 20% fetal bovine serum and 10% DMSO
Culture conditions:Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing instructions:Cell Culture Procedures


Characterized By:
  • Establishment and characterization of a novel chordoma cell line: CH22. Liu X, Nielsen GP, Rosenberg AE, Waterman PR, Yang W, Choy E, Sassi S, Yang S, Harmon DC, Yang C, Schwab JH, Kobayashi E, Mankin HJ, Xavier R, Weissleder R, Duan Z, Hornicek FJ. J Orthop Res. 2012 Oct;30(10):1666-73. doi: 10.1002/jor.22113.