Chordoma Foundation

U-CH2

General Characteristics


Age: 72 years
Gender: Female
Tumor Location: Sacral
Tumor Status: Recurrent
Source: Silke Bruderlein, Peter Moller
University of Ulm

 

Molecular Features


Cell morphology: Physaliferous
T expression: Positive; with T amplification
T localization: Nuclear
CD24 expression: Positive
HLA type: A11 View Histocompatibility Report
Chromosomal abnormalities: Multiple, including amplification of T/brachyury and biallelic loss at 9p21 (CDKN2A and CDKN2B loci)
Sequence data: WGS, Exome and Transcriptome data available contact patty@chordoma.org
Validation results: Validation Report

 

Growth Conditions


Growth medium: IMDM: RPMI-1640 (4:1) +10% FBS
Freeze medium: 70% complete growth medium supplemented with an additional 20% fetal bovine serum and 10% DMSO
Culture conditions: Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing instructions: Cell Culture Procedures

 

Publications


Characterized By:

  • Brüderlein S, Sommer J, Meltzer P, Li S, Osada T et al. (2010) Molecular Characterization of Putative Chordoma Cell Lines. Sarcoma: 630129.  PubMed: 21253487.

Additional References:

  • Scheil-Bertram S, Kappler R, von Baer A, Hartwig E, Sarkar M et al. Molecular profiling of chordoma. Int J Oncol 44(4):1041-55. PubMed: 24452533.
  • Xia M, Huang R, Sakamuru S, Alcorta D, Cho MH et al. (2013) Identification of repurposed small molecule drugs for chordoma therapy. Cancer Biol Ther 14(7):638-47. PubMed: 23792643.

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