Chordoma Foundation

JHC7

General Characteristics


Age: 61 years
Gender: Female
Tumor Location: Sacral
Tumor Status: Primary
Source: Sagar Shah and Alfredo Quiñones-Hinojosa
Johns Hopkins University

 

Molecular Features


Cell morphology: Physaliferous
T expression: Positive; 9.2x that of U-CH2 and with T amplification
T localization: Nuclear
CD24 expression: Positive; 6.4x that of U-CH2
HLA Type:  A24
Chromosomal abnormalities: Multiple, including amplification of T/brachyury
 Sequence data:  WGS and Transcriptome data available contact patty@chordoma.org
Validation results: Validation Reports

 

Growth Conditions


Growth medium: DMEM/F12+10%FBS+glutamine
Freeze medium: 70% complete growth medium supplemented with an additional 20% fetal bovine serum and 10% DMSO
Culture conditions: Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing instructions: Cell Culture Procedures

 

Publications


Characterized By:

  • Hsu W, Mohyeldin A, Shah SR, ap Rhys CM, Johnson LF et al. (2011) Generation of chordoma celll line JHC7 and the identification of Brachyury as a novel molecular target. J Neurosurg 115(4): 760-9. PubMed: 21699479.

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